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bis_104_pq_12_ans_fall_08

bis_104_pq_12_ans_fall_08 - directed against VEGF(red and...

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BIS 104 Practice Question 12 12. Mukouyama et al. claim to have experimental evidence that VEGF is expressed in nerves at a relatively higher level than in surrounding mesenchymal tissue. Describe the experiment and the results they obtained that tend to that support this claim. EXPERIMENT: See fig. 6 color panels A, B, & C. 1. Skin samples from E14.5 mouse limbs were stained with fluoresently labeled antibodies
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Unformatted text preview: directed against VEGF(red) and against neurons (green - TuJ1 marker for tubulin). 2. Stained samples were examined by double immunofluorescence confocal microscopy. 3. The staining pattern of the two markers was compared. RESULTS: VEGF is expressed in nerves at a relatively higher level than in surrounding mesenchymal tissue, as seen from the intensity of fluorescence from the two markers ....
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