Practice%20Exam%201

Practice%20Exam%201 - BIBC 103 Practice Exam 1 1 For a...

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BIBC 103 Practice Exam 1 1) For a laboratory project you asked to purify the enzyme lactate dehydrogenase (LDH). The first step of this project is to prepare the lysis buffer that you will use to extract the enzyme. The protocol you are following says the LDH lysis buffer should contain: 25 mM CAPS (a pH buffer) at pH 10 5 mM MgCl 2 0.2% Triton X-100 (a detergent) 10 μ g/ml pepstatin A (a protease inhibitor) You find the following reagents on the shelves in lab: a bottle of CAPS powder; MW is 221.3, pKa is 10.4 a bottle of MgCl 2 crystals; MW is 95.2 a bottle of Triton X-100 (this detergent is a liquid in its pure state) and a stock solution of 25 mg/ml pepstatin A You need to make 200 ml of this buffer. Tell how you would make this solution. Show your work. 2) A weak acid compound can dissociate a single hydrogen ion with a pKa value of 3.7. Use the Henderson-Hasselbalch equation to calculate the ratio of proton acceptor form (A-) over the proton donor form (HA) at a pH value of 4.2.
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This note was uploaded on 01/27/2009 for the course BIBC bibc 103 taught by Professor Coleman during the Winter '09 term at UCSD.

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Practice%20Exam%201 - BIBC 103 Practice Exam 1 1 For a...

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