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Lab 7 - M Farrell Matt Farrell November 3 2006 Lab 7...

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M. Farrell Matt Farrell November 3, 2006 Lab 7: Isolation of Caffeine by Column Chromatography Purpose: The purpose of this lab is to extract caffeine using column chromatography. The isolation of caffeine is aided by a column chromatograph filling the tube with glass wool, a layer of sand, silica gel, and another layer of sand to help separate the caffeine. Background: Column chromatography is used for the practice of separating compounds by partitioning them between a mobile liquid phase and a stationary solid phase that is packed in a glass column. The mixture is applied at the top of the column and is washed through the column by a pure solvent. The mixture then moves over the solid phase, the compounds move apart in discrete zones. The separated compounds exit the column and are collected. Usually the solvent is evaporated and compounds are further purified and identified. During this experiment caffeine will be extracted from tea using a column chromatograph.
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