Lab 5 - M. Farrell Matt Farrell October 15, 2006 Lab 5:...

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M. Farrell Matt Farrell October 15, 2006 Lab 5: Introduction to Chromatography Purpose: The purpose of this lab was to determine the caffeine content of a beverage sample. Another purpose of the lab is to be proficient in using two types of liquid chromatography, high performance liquid chromatography, and thin layer chromatography. When using these techniques, it is required to extract caffeine from a certain beverage. Background: Liquid chromatography is used to separate and purify organic compounds. HPLC has the capacity to be used analytically and to prepare to separate mixtures of close eluting compounds. An absorbance detector measures the absorbance of a particular wavelength of ultraviolet light, usually for aromatic compounds. TLC’s most common use is to monitor reactions. For example, the disappearance of starting material and appearance of the products. TLC chamber Page 1 of 5
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M. Farrell Procedure: 1. Gather stand, with ring and 125 mL separatory funnel. 2. Add 40 mL of Dr. Pepper to funnel. 3.
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This lab report was uploaded on 04/15/2008 for the course CHEM 333 taught by Professor Koh during the Fall '06 term at University of Delaware.

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Lab 5 - M. Farrell Matt Farrell October 15, 2006 Lab 5:...

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