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Unformatted text preview: Which one of the following is a likely reason why? Explain. A. Not enough template DNA was used in the reaction. B. Too much primer DNA was used in the reaction. C. Too much dideoxy nucleotide was added to the reaction. D. Too little dideoxy nucleotide was added to the reaction. 5. You wish to determine the sequence similarity for DNA from three different species A, B and C). DNA from each of the species is denatured, mixed with the DNA from another species and allowed to reanneal. You then determine the melting point of each of the hybrids formed. Which two species have the greater degree of similarity in their DNA sequences? Why? Hybrid Duplex Melting Point A + B 88 ° C A + C 84 ° C B + C 78 ° C...
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This note was uploaded on 04/15/2008 for the course BIO SCI 98 taught by Professor Goulding during the Spring '08 term at UC Irvine.
- Spring '08