BS9907TakeHomeQuiz - Which one of the following is a likely...

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BS99 Take Home Quiz Name _________________________________ Student ID ________________________ 1. Bacteriophage M13 infects E. coli differently from the way bacteriophage T2 does. The M13 coat is removed in the inner membrane of the bacterial cell, where it is sequestered during phage replication. It is subsequently used to package the newly replicated phage DNA to create progeny phage. Why would this make M13 less suitable than T2 for the Hershey and Chase experiment? 2. Draw the complementary strand for the following DNA sequence.
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3. When thymine is in the lactim form (looks just like uracil but with the methyl group) it can base pair with guanine. Draw the base pair formed by the two bases demonstrating the likely hydrogen bonds formed. 4. A sequencing reaction resulted in many more shorter fragments than longer ones.
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Unformatted text preview: Which one of the following is a likely reason why? Explain. A. Not enough template DNA was used in the reaction. B. Too much primer DNA was used in the reaction. C. Too much dideoxy nucleotide was added to the reaction. D. Too little dideoxy nucleotide was added to the reaction. 5. You wish to determine the sequence similarity for DNA from three different species A, B and C). DNA from each of the species is denatured, mixed with the DNA from another species and allowed to reanneal. You then determine the melting point of each of the hybrids formed. Which two species have the greater degree of similarity in their DNA sequences? Why? Hybrid Duplex Melting Point A + B 88 ° C A + C 84 ° C B + C 78 ° C...
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