BS9907PS2Key - BIO SCI 99 Problem Set#2 1 Heparin is a...

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BIO SCI 99 Problem Set #2 1. Heparin is a polyanion that inhibits RNA transcription in vitro by binding to the β ’ subunit of bacterial RNA polymerase (RNAP). What function of RNAP is heparin interfering with? Non-specific binding of the RNAP to the DNA. 2. The 5' end of the coding strand of a prokaryote gene is diagramed below. Answer the following questions based on the sequence information. +1 GACATAAACCCTTTGGGTTGACA (N) 17 GCTATAAT (N) 7 A GTGGGAGGAGCCCGTGGGGACATG GAACCC σ Pribnow start a. Underline the following sequences: the σ binding site, the pribnow box, the translational start site b. If an additional 10 nucleotides were inserted into the (N) 17 region would you expect RNAP to initiate transcription efficiently at this site? No, consensus position is as important as consensus sequence to the RNAP. 3. A mutant of E. coli has constitutive expression of β -galactosidase. A partial diploid formed with this mutant and F I + O + Z + has inducible synthesis of β -galactosidase. What is the genotype
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BS9907PS2Key - BIO SCI 99 Problem Set#2 1 Heparin is a...

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