BS9907PS1Key - Bio Sci 99 Problem Set #1 1. A viral DNA is...

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1. A viral DNA is analyzed and found to have the following base composition: G = 32%, A = 16%, C = 40%, T = 12%. a). What can you immediately conclude about this DNA? Since G is not equal to C and A is not equal toT then Chargaff’s rules for double- atranded DNA are not being followed. The DNA is not double-stranded and is therefore single-stranded. b). What kind of secondary structure do you think it would have? It is possible for this DNA to have some secondary structure such as hairpin and stem loops. 2. Given the following sequence for one strand of a double-stranded oligonucleotide: 5’ ACCGTAAGGCTTTAG 3’ a). Write the sequence for the complementary strand. 3’ TGGCATTCCGAAATC 5’ b). Write the sequence of the RNA complementary to the sequence given. 3’ UGGCAUUCCGAAAUC 5’ or 5’ CUAAAGCCUUACGGU 3’ 3. The following polynucleotide sequence is palindromic: TC AAGTCCATGGACTT GG AG TTCAGGTACCTGAA CC Show how this structure might form a cruciform conformation. T A
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This note was uploaded on 04/15/2008 for the course BIO SCI 98 taught by Professor Goulding during the Spring '08 term at UC Irvine.

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BS9907PS1Key - Bio Sci 99 Problem Set #1 1. A viral DNA is...

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