biolabreport - The Effects of Calcium and Sodium on Amylase...

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1 The Effects of Calcium and Sodium on Amylase Activity in Barley Seed Germination Shannon Stoufer, MCDB 1BL Tues. 4-7pm Introduction This experiment was performed in order to investigate what the effect is of calcium and sodium on gibberellic acid and amylase production during the germination cycle of a barley seed. The exercise of regulation of physiological processes introduced the concept of basic cereal grain germination and the function of amylase production. In the aleurone tissue, GA (gibberellic acid) is a hormone that induces the cells to produce alpha-amylase in order to break down glucose in the endosperm(Kaska and Bush, 2008). It is known that calcium is important for the response of aleurone to GA, however, to what degree it is a factor in the cells responses is under examination.(Bush, 1996) Furthermore, the presence of external sodium and salt stress is a factor in inhibition of amylase production, but its interactions and control are also questionable (Kaur, S., Gupta, Kaur, N., 1998) This led to seed tissue for amylase activity under the effect of different concentrations in calcium and sodium. Methods Six incubation mediums were prepared and transferred to the six starch agarose plates. The mediums were composed of GA (10 F M) , either CaCl 2 (1mM, 30mM, 100mM) or NaCl (1mM, 30mM, 100mM), and water to make the solutions 3ml. After autoclaving, the incubation mediums were poured into separate plates and then 10 half barley seeds were added. The plates were then stored for at least 48 hours to incubate seeds before measuring amylase production. After one week the petri dishes were retrieved to test amylase activity. Activity was measured using spectrophotometric techniques. Water (6mL) and iodide reagent (1mL) was mixed in a test tube and ran through the spectrophotometer in order to get the maximum absorbance value. The amylase assays were prepared with incubation medium (25 F l) of treatment being tested and .975 mL water. To stop the reaction after a measured time interval iodide reagent (1mL) was added, followed by water (5 mL). The assay was then ready to put in the spectrophotometer for absorbance reading. Results
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This note was uploaded on 04/15/2008 for the course MCDB 1B taught by Professor Weimbs during the Winter '08 term at UCSB.

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biolabreport - The Effects of Calcium and Sodium on Amylase...

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