Worksheet 9 - in vivo . b. _________ (DNA/RNA) primers are...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO 325 GENETICS: Worksheet # 9 (Wednesday March 26, 2008) 1. At what stage of cell cycle does DNA replication occur and where in the cell? 2. What are the three models proposed to explain the mechanism of DNA replication? Which one is true for most of the organisms? 3. True or False: a. Only DNA polymerase I is found in E. coli. b. Both DNA polymerase and RNA polymerase can start de novo . c. DNA helicase unwinds dsDNA during the first cycle of PCR. 4. A gene that is needed to make pink pigment in plants is mutated. The mutant still makes pink flowers. How would you explain this result? 5. How is 3’-5’ exonuclease different from 5’-3’ exonuclease? 6. What are primers and why are they needed in a DNA replication process? 7. Fill in the blanks: a. _________ (DNA/RNA) primers are needed to start DNA replication
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: in vivo . b. _________ (DNA/RNA) primers are needed to start DNA replication in a PCR reaction. c. DNA polymerase adds nucleotides in ____________ direction. d. Initiation of DNA replication involves binding of ________ proteins to dsDNA. e. ________________ enzyme removes primers from template DNA by its _________ activity. 8. What is the importance of having an exonuclease activity during DNA replication? 9. Between which subunits of a nucleotide are the following bonds made: a. Covalent bond b. Phosphodiester bond c. Hydrogen bond 10. Label the leading and lagging strand in the following picture: 11. How does Topoisomerase alleviate positive supercoiling? 12. Label the empty boxes in the following picture of DNA replication: 3’ 5’ 5’ 3’ 6 4 3 2 1 7 5...
View Full Document

This homework help was uploaded on 04/16/2008 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

Page1 / 2

Worksheet 9 - in vivo . b. _________ (DNA/RNA) primers are...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online