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Worksheet 9 - in vivo b(DNA/RNA primers are needed to start...

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BIO 325 GENETICS: Worksheet # 9 (Wednesday March 26, 2008) 1. At what stage of cell cycle does DNA replication occur and where in the cell? 2. What are the three models proposed to explain the mechanism of DNA replication? Which one is true for most of the organisms? 3. True or False: a. Only DNA polymerase I is found in E. coli. b. Both DNA polymerase and RNA polymerase can start de novo . c. DNA helicase unwinds dsDNA during the first cycle of PCR. 4. A gene that is needed to make pink pigment in plants is mutated. The mutant still makes pink flowers. How would you explain this result? 5. How is 3’-5’ exonuclease different from 5’-3’ exonuclease? 6. What are primers and why are they needed in a DNA replication process? 7. Fill in the blanks: a. _________ (DNA/RNA) primers are needed to start DNA replication
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Unformatted text preview: in vivo . b. _________ (DNA/RNA) primers are needed to start DNA replication in a PCR reaction. c. DNA polymerase adds nucleotides in ____________ direction. d. Initiation of DNA replication involves binding of ________ proteins to dsDNA. e. ________________ enzyme removes primers from template DNA by its _________ activity. 8. What is the importance of having an exonuclease activity during DNA replication? 9. Between which subunits of a nucleotide are the following bonds made: a. Covalent bond b. Phosphodiester bond c. Hydrogen bond 10. Label the leading and lagging strand in the following picture: 11. How does Topoisomerase alleviate positive supercoiling? 12. Label the empty boxes in the following picture of DNA replication: 3’ 5’ 5’ 3’ 6 4 3 2 1 7 5...
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