Lab 3 - Caitlin Grimshaw BIOL 216-703 Bacterial Morphology...

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Caitlin Grimshaw BIOL 216-703 Bacterial Morphology Lab #3 Purpose The purpose of this lab is to test our ability to see defining characteristics of bacteria and other microbes which is called morphology, by preparing wet-mount slides and learning direct and indirect staining techniques. Procedure Wet mount slides: First I started out by viewing the prepared slides of bacterial morphology I then disinfected my work space. I prepared my three slides using the marker pencil to make dime-sized circles on them. Taking the pipet I added water to the circle of the first slide. Then using a cotton swab I scraped the inside of my mouth and smeared the swab on the circle of the first slide. I prepared the second slide in the same manner but this time used a toothpick and scraped a sample of plaque from my teeth. And finally with the third slide I added a drop the S. cerevisiae mixture to the circle. Direct staining: Just like in the wet mount slides I prepared three slides with dime-sized marks. Except this time on the first slide add yeast to the circle. Slide two and three are the exact same at mentioned above. Leave all three slides to dry, then heat-fit each slide Indirect staining: Once again prepare the slides with three marker circles. One the first slide add a drop of Congo red to the circle. Doing the same thing to slide two but proceeding to add the sample from the toothpick. Adding the Congo red to the third slide, take the cotton swab and scrap the inside of your mouth and rolling it in the Congo red. Data Tables Exercise 1: Prepared Slides Prepared slides 10X 40X 100X Cocci Hard to see Tiny cells Able to see the nucleus in the center Bacillus Tiny dot Dots look more like shapes Tiny rods
Spirillum Tiny black spot Black lines Spiral shaped cells with visible nucleus Exercise 2: Wet Mount Preparations Preparation 10x 40x 100x Mouth Swab Several shaped cells Varied different cells Plaque Dark circular shapes Yeast Odd shaped Very distinct edges Exercise 3: Direct Staining Preparation 10x 40x 100x Mouth Swab Two clusters were recognized

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