The purpose of this lab is to test our ability to see defining characteristics of bacteria and other
microbes which is called morphology,
by preparing wet-mount slides and learning direct and
indirect staining techniques.
Wet mount slides:
First I started out by viewing the prepared slides of bacterial morphology I then disinfected my
work space. I prepared my three slides using the marker pencil to make dime-sized circles on
them. Taking the pipet I added water to the circle of the first slide. Then using a cotton swab I
scraped the inside of my mouth and smeared the swab on the circle of the first slide. I prepared
the second slide in the same manner but this time used a toothpick and scraped a sample of
plaque from my teeth. And finally with the third slide I added a drop the S.
Just like in the wet mount slides I prepared three slides with dime-sized marks. Except this time
on the first slide add yeast to the circle. Slide two and three are the exact same at mentioned
above. Leave all three slides to dry, then heat-fit each slide
Once again prepare the slides with three marker circles. One the first slide add a drop of Congo
red to the circle. Doing the same thing to slide two but proceeding to add the sample from the
toothpick. Adding the Congo red to the third slide, take the cotton swab and scrap the inside of
your mouth and rolling it in the Congo red.
Exercise 1: Prepared Slides
Hard to see
Able to see
nucleus in the center
Dots look more like