Prelab for Spectrophotometric Determination of Multi-Component Solutions - Prelab for Spectrophotometric Determination of Multi-Component Systems

Prelab for Spectrophotometric Determination of Multi-Component Solutions

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Prelab for Spectrophotometric Determination of Multi-Component Systems Chemisty 112L Section 001 September 30, 2014 1. Interference is when more than one species absorbs at the same wavelength. The closer the two curves are to one another, the greater the interference. 2. 6.2 g 2 qt 1 qt 946.352946 mL 100 mL 1 = 0.32757 g When rounded to the correct number of significant figures 0.33 g of powder would need to be weighted out to make 100.0 mL of solution with the same concentration. 3. The variables plotted on the x and y axes in a Beer’s Law graph are concentration and absorbance respectively. The slope of this graph provides the molar absorptivity of the solution being graphed. 4. I will use the two equation method presented in the introduction of the prelab to simultaneously solve for the two unknown concentrations. | ( λmax A ) | = ε A ( λmax A ) C A + ε B ( λmax A ) C B 0.75 =( 7.8 M 1 cm 1 ) C A +( 1.5 M 1 cm 1 ) C B | ( λmax B ) | = ε A ( λmax B ) C A + ε B ( λmax B ) C B 0.45 =( 2.6 M 1 cm 1 ) C A +( 5.2 M 1 cm 1 ) C B

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