JV6 KEY - Electron Transport Chain & Oxidative...

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1. What are two major structures that can make a protein a conductor of electrons? Answer to this is located in WORKSHEET 5 2. Understand the significance of these complexes. Fill in the table. Complex Function Overall Reaction # of H+ Pumped from Matrix to IMS Answer to this chart is located in WORKSHEET 5 I. ATP Synthase (Complex V) 1. Mitchell’s “Chemiosomatic Hypothesis”. Energy released from the oxidation of NADH through ETC is used to drive ATP synthesis. Draw a schematic diagram and describe the main ideas of this hypthesis. - NADH is oxidized in Complex I - electrons are transported to O2 via several intermediates and mobile carriers - complex I, III, IV pump H+ from matrix to IMS via active transport provides membrane potential and H+ gradient - H+ comes back into matrix via complex V
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- This forces electrons of H+ returning from high to low concentration is used to drive ATP synthesis ATP synthase rotary mechanism rotation is used for formation of ATP from ADP + Pi 3H+ and 120 degrees turn of ATP synthase produce 1 ATP note: ATP synthase and ETC are completely separate, they are independent of each other. they can be uncoupled by alternative routes for H+ 2. ATP synthase: a. Draw a schematic structure of the ATP synthase with all the essnetial subunits. Explain how rotary motion is responsible for the synthesis of ATP. the alpha and beta subunits are FIXED, only the y and F0 rotate beta subunit of F1 changes conformation as y subunit associates with each turn H+ motive force causes rotation of Y subunit Y comes into contact with each alpha/beta subunit pair
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This note was uploaded on 04/15/2008 for the course BIBC 102 taught by Professor Price during the Winter '02 term at UCSD.

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JV6 KEY - Electron Transport Chain & Oxidative...

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