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Unformatted text preview: BIBC 102- Metabolic Biochem Jacqueline Vu 3/12/08 Worksheet #9 http://groups.google.com/group/ bibc102jackie Please Fill out Evaluations http://biology.ucsd.edu/genericsurvey/studenttaeval.jsp ♫ ♫ ♫ ♫ ♥ ♥ ♫ ♫ ♫ ♫ ♪ ♪ ♪ ♥ ♥ ♥ ♥ ♫ ♫ ♫ ♫ ♫ FA Synthesis 1. Where does FA synthesis take place? 2. A starting material for FA synthesis is Acetyl-CoA . It will then be converted to malonyl-CoA by enzyme acetyl-Coa carboxylase . This enzyme is a single polypeptide with 3 different activities: biotin carrier protein, biotin carboxylase , and transcarboxylase . At the biotin carboxylase region, ATP is involved to the attachment of HCO 3- to a nitrogen in the biotin ring. At the transcarboxylase region , the activated CO 2 is transferred from biotin to acetyl-CoA , producing malonyl-CoA . General Equation: 3. Both acetyl-CoA and malonyl-CoA are substrates for the fatty acid synthase. Describe with structural formula the steps required to make the first 4C molecules in the process of FA synthesis 1 BIBC 102- Metabolic Biochem Jacqueline Vu 3/12/08 Worksheet #9 http://groups.google.com/group/ bibc102jackie Please Fill out Evaluations http://biology.ucsd.edu/genericsurvey/studenttaeval.jsp ♫ ♫ ♫ ♫ ♥ ♥ ♫ ♫ ♫ ♫ ♪ ♪ ♪ ♥ ♥ ♥ ♥ ♫ ♫ ♫ ♫ ♫ In general, it is a 4-step sequence to add 2C to a growing fatty acyl chain: a. Condenstaion of an activated acyl group and 2C derived from malonyl- CoA with elimination of CO 2 from malonyl group, extends acyl chain by 2C b. Reduction : beta-keto group is reduced to an alcohol c. Dehydration of H 2 0 creates a double bond d. Reduction : double bond is reduced to form the corresponding saturated fatty acyl group. 4. Radioactive 14 CO 2 was used in the production of malonyl-CoA in a bacterial extract. Could this radioactive starting material now be used to make radioactive fatty acids by an animal FA synthase? Explain briefly. No, CO 2 leaves after the first condensation reaction of FA synthesis....
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This note was uploaded on 04/15/2008 for the course BIBC 102 taught by Professor Price during the Winter '02 term at UCSD.
- Winter '02