Protocol Absorbance spectrum

Protocol Absorbance spectrum - Rebecca Kennedy Protocol Lab...

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Rebecca Kennedy Protocol Lab Ex #10 Photosynthesis: Absorption Spectrum Purpose: The purpose of the absorption spectrum experiment is to determine the wavelengths most useful for photosynthesis by determining the absorption spectrum of a pigment extract. Hypothesis: When pigment is combined with acetone and placed in a cuvet and acetone is used as a blank the absorption spectrum of each pigment will be able to be determined. Null Hypothesis: When pigment is combined with acetone and placed in a cuvet and acetone is used as a blank the absorption spectrum of each pigment will not be able to be determined. Preditions: If the yellowish pigment from Carotene is tested for absorption spectrum it will be determined to be between 560 and 580nm. If the yellowish pigment from Xanthophylls is tested for its absorbance spectrum it will be determined to be between 560 and 580nm. If the greenish pigment from chlorophyll a is tested for its absorbance spectrum it will be determined to be between 525 and 560nm. If the bluish green pigment from chlorophyll b is tested for its absorbance spectrum it will be determined
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This note was uploaded on 04/15/2008 for the course BIOSC Bio Lab taught by Professor Karen during the Spring '08 term at Pittsburgh.

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Protocol Absorbance spectrum - Rebecca Kennedy Protocol Lab...

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