Protocol Photosynthesis

Protocol Photosynthesis - be larger in size If Chlorophyll...

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Rebecca Kennedy Purpose: The purposes identify the pigments involved in photosynthesis by paper chromatography and understand chlorophyll and the accessory pigments and finally determine the wavelengths most useful for photosynthesis by determining the absorption spectrum of a pigment extract. Hypothesis: If pigments are extracted from spinach leaves and then applied to chromatography then the mixture will separate into several components at the end of the chromatography. Null Hypothesis: If pigments are extracted from spinach leaves and then applied to chromatography then the mixture will not separate into several components. Predictions: If Carotene (carotenoid) separates from the mixture as a separate component then it will appear yellow in color and will be the smallest in size. If Xanthophylls (carotenoid) separates from the mixture as a separate component then it will appear yellowish and may have 1 or 2 bands. If Chlorophyll a separates from the mixture as a separate component then it will appear greenish and will
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Unformatted text preview: be larger in size If Chlorophyll b separates from the mixture as a separate component then it will appear bluish green and largest. Rationales: If Carotene (carotenoid) separates from the mixture as a separate component then it will appear yellow in color and will be the smallest in size because it is the most non-polar hence, the most soluble. If Xanthophylls (carotenoid) separates from the mixture as a separate component then it will appear yellowish and may have 1 or 2 bands because it is les non-polar than carotene. If Chlorophyll a separates from the mixture as a separate component then it will appear greenish and will be larger in size because it is less non-polar than carotene and xanthophylls If Chlorophyll b separates from the mixture as a separate component then it will appear bluish green and largest because it is the least non polar hence, the least soluble....
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This note was uploaded on 04/15/2008 for the course BIOSC Bio Lab taught by Professor Karen during the Spring '08 term at Pittsburgh.

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Protocol Photosynthesis - be larger in size If Chlorophyll...

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