Ch 10t - Chapter 10: Accessing Genomes Molecular Genetics...

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Chapter 10: Accessing Genomes Molecular Genetics Lecture Outline Tech Note 10.1: Fluorescence recovery after photobleaching (FRAP). Make a cell line transgenic for a target protein fused to green fluorescent protein (GFP). The cells fluoresce all over under UV light. Hit a spot inside the cell (in this case, inside the nucleus) with a laser beam and it bleaches the GFP. The GFP at that spot doesn’t recover, but other molecules of the tagged protein move into the spot. The rate at which the spot recovers, then, is related to the intrinsic motility of your target protein at that place in the cell. Answers the question “Do proteins move around or do they just sit there?”. Fig. 10.2: In interphase, the chromatin is dispersed instead of being tightly condensed as it is in the familiar metaphase chromasome structures. It is found as 30 nm chromatin fibers (Fig. 10.5). What is heterochromatin? What is the difference between facultative and constitutive forms? What is euchromatin?
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Ch 10t - Chapter 10: Accessing Genomes Molecular Genetics...

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