Bacterial transformation lab (1) (1).docx - Bacterial...

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Bacterial Transformation Part 1Purpose and BackgroundIn this lab, you will perform a procedure known as genetic transformation.Transformation is defined as the insertion of a gene into an organism in orderto change the organism’s trait(s).Genetic transformation is used in many areas of biotechnology.In medicine, gene therapy involves transforming a sick person’s cellswith healthycopies of the defective gene that causes thedisease.In research, bacteria are transformed with genes encoding humanproteins forbiomanufacturing production or for furtherstudy of these proteins.In agriculture, genes coding for traits such as frost-, pest-, or spoilage-resistance canbe genetically transformed into plants.In bioremediation, bacteria can be transformed with genes that enablethem toWe will be transforming bacteria with a gene that codes for GreenFluorescent Protein (GFP).This gene originally came from the bioluminescentjellyfishAequorea Victoria.GFP is the protein that causes this jellyfish to glowin the dark.If your transformation is successful, the transformed bacteriaexpressing the GFP protein will glow a brilliant green color under ultravioletlight.75digest oil spills.
Before beginning the laboratory activities, we will first learn morebackground on transformation in general and our particular experiment in aPowerpoint lecture (see next 4 pages for notes).

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Term
Summer
Professor
Farrell

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