- The Domains of Life Bacteria Archaea Eukarya Lecture by Avelina Espinosa PhD Current estimates of microorganisms community diversity 160

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2/11/2008 1 The Domains of Life: Bacteria, Archaea, Eukarya Lecture by: Avelina Espinosa, PhD Current estimates of microorganisms community diversity • 160 species/ml of seawater Æ 10 29 cells • 6,400 - 38,000 species/gram of soil • Human gut Æ 10 14 cells Curtis et al. (2002) PNAS Ley (2006) Cel Environmental sampling rRNA – shared by all organisms – universal PCR primers t b li di it i f – no metabolic diversity info Random genomic DNA – mixed DNA samples – “fishing” method – genes Æ metabolic diversity rRNA phylogeny rRNA tree Pace NR (1997) Science 276, 734
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2 Bacteria & Eukarya Æ Ester linkages between glycerol & fatty acid side chains Archaea: Ether linkages Æ cell membrane different, hydrocarbon side chains (ancestral membrane composition) Domain Bacteria Domain Archaea Domain Eukarya Proteobacteria Alpha Beta Gamma Delta Epsilon Chlamydias Spirochetes Cyanobacteria Gram-positive bacteria Korarchaeotes Euryarchaeotes Crenarcaeotes Nanoarchaeotes Eukaryotes Universal ancestor Bacteria Energy sources in Bacteria Chemolithotrops = oxidation CO
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This note was uploaded on 04/16/2008 for the course BIO 131 taught by Professor Paz y mino during the Fall '08 term at UMass Dartmouth.

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- The Domains of Life Bacteria Archaea Eukarya Lecture by Avelina Espinosa PhD Current estimates of microorganisms community diversity 160

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