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chapter 27 28 - The Domains of Life Bacteria Archaea...

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2/11/2008 1 The Domains of Life: Bacteria, Archaea, Eukarya Lecture by: Avelina Espinosa, PhD Current estimates of microorganisms community diversity 160 species/ml of seawater Æ 10 29 cells 6,400 - 38,000 species/gram of soil Human gut Æ 10 14 cells Curtis et al. (2002) PNAS Ley et al. (2006) Cell Environmental sampling rRNA shared by all organisms universal PCR primers no metabolic diversity info Random genomic DNA mixed DNA samples “fishing” method genes Æ metabolic diversity rRNA phylogeny rRNA tree Pace NR (1997) Science 276, 734
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