This preview shows page 1. Sign up to view the full content.
Unformatted text preview: caused an increase in endopeptidase activity (indirect indicator of the number of sperm) in samples taken from the upper chamber. Control: Fill upper compartment with buffer only (no follicular fluid) . Measure endopeptidase activity as in experiment. OR Fill both upper and lower compartments with the same concentration of follicular fluid. Measure endopeptidase activity at 15 minutes as in experiment. 3. EXPERIMENT: Dilute sperm into NSW and add A23187 (calcium ionophore). Observe by microscopy to determine if AMF part of AR has been triggered. RESULTS: AMF has occurred. APF has not occurred. CONTROL: Place sperm in CaFSW; add same concentration of A23187 as in experiment. Observe for AMF by microscopy....
View Full Document
This note was uploaded on 04/16/2008 for the course MCB 150 taught by Professor Armstrong,peter during the Fall '07 term at UC Davis.
- Fall '07