b101fn06a - NAME: ID# Last, First BIS101-2 Winter 2006...

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NAME: ID# Last, First BIS101-2 Winter 2006 FINAL EXAM SHOW ALL WORK TO GET FULL CREDIT . I,________________________ , ID#(6 last digits)_________________________ authorize posting of my grade in Briggs (1st floor) by the 6 last digits of my ID# Note: Final exams will not be returned and no re-grading petitions will be honored . Only claims for clerical errors (errors adding points or transferring grades to roster) will be accepted. The exams will be only available for inspection by appointment until April 15th at 116 Asmundson. 1
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QUESTION VALUE SCORE 1 10 2 20 3 10 4 20 5 27 6 23 7 25 8 22 9 16 10 15 11 12 TOTAL 200 2
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1. (10 pts) Match the names to the discoveries : ____4 Jacob and Monod 1. Linkage maps ___7_ Benzer 2. Trp operon/attenuation ___9 Morgan 3. Recombinant DNA ___10 Holliday 4. Lac operon ___1 Sturtevant 5. Proof of chromosome theory ___5_ Bridges 6. DNA semi-conservative replication ___6__Meselson-Stahl 7. Cistron ___8__Beadle and Tatum 8.One gene one enzyme ___2__Yanofsky 9. Sex Linkage ____3__ Cohen and Boyer 10. Model for recombination 2. (20 pts) The pBR322 and pUC19 plasmids are used as a vectors in recombinant DNA procedures to clone genes. pBR322 carries tetracycline and ampicillin resistance genes used for selection, whereas pUC19 carries an ampicillin resistance gene and a LacZ’ gene for selection. In independent experiments, a gene coding for human lactoferrin was inserted into a restriction site in the polylinkers located inside the tetracycline gene in pBR322, and in the LacZ gene in pUC19. Then the plasmid were then introduced by transformation into E. coli cells. Describe the procedures you will follow in each case to select the bacterial colonies carrying the recombinant plasmids, including media composition. pBR322: Growth the bacteria after transformation in a master plate containing antibiotic free medium. Replicate plate in 2 plates. The first one containing ampicillin to select for transformed bacteria, and the second one containing both ampicillin and tetracycline. Colonies containing recombinant DNA plasmids will not grow in the plate since the tetracycline resistant gene has been disrupted by the introduction of the lactoferrin gene. Therefore these can be selected from the master plate. pUC19: Grow bacteria after transformation in a plate containing ampicillin and the lactose analog such as x-gal/IPTG that turns blue when the LacZ’ gene is functional. Select the white colonies which are the ones carrying the recombinant DNA plasmid since they have a non-functional LacZ’gene due to disruption by the lactoferrin gene. Therefore they are unable to produce blue color. 3
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3) (10 pts) Two inbred varieties of morning glory developed by different seed companies have white flowers. All other varieties of this species have blue flowers. There are 2 loci involved in the synthesis of flower color in this species. White is recessive to blue color. Design a simple experiment to determine whether white flower color is caused by
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b101fn06a - NAME: ID# Last, First BIS101-2 Winter 2006...

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