Bis101_Quiros_Fall2002_Final

Bis101_Quiros_Fall2002_Final - BIS101-01 FINAL EXAM ID Fall...

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BIS101-01 FINAL EXAM Fall 2002 NAME: ID# Last First (last 6 digits) Authorization for grade posting by 6 last digits of SS# I,________________________ , ID#(6 last digits)_________________________ authorize posting of my grade in Briggs (1rd floor) by the 6 last digits of my ID# Note: Final exams will not be returned and no re-grading petitions will be honored . Only claims for clerical errors (errors adding points or transferring grades to roster) will be accepted. The exams will be only available for inspection by appointment (from Dec 18-21,contact Bo Yang ( [email protected] ). From Dec 23- Jan 10th come to 116 Asmundson. SHOW ALL WORK TO GET FULL CREDIT .
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FINAL F’02 QUESTION VALUE SCORE 1 20 2 20 3 24 4 20 5 20 6 20 7 20 8 15 9 25 10 12 TOTAL 200
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1. (a) Tomatoes have a diploid (2n) number of 24 chromosomes. Identify the number of chromosomes that would be expected in a tomato plant that is: 1. 23 a monosomic. (2) 2. 25 a trisomic. (2) 3. 24 a heterozygote for a reciprocal translocation. (2) 4. 22 a nullisomic. (2) 5. 60 a pentaploid. (2) (b). (10 pts) Match the names to the discoveries : ____4 Jacob and Monod 1. Linkage maps ___7_ Benzer 2. Trp operon/attenuation ___9 Morgan 3. Recombinant DNA ___10 Holliday 4. Lac operon ___1 Sturtevant 5. Proof of chromosome theory ___5_ Bridges 6. DNA semi-conservative replication ___6__Meselson-Stahl 7. Cistron ___8__Beadle and Tatum 8.One gene one enzyme ___2__Yanofsky 9. Sex Linkage ____3__ Cohen and Boyer 10. Model for recombination 2. (20 pts) The pUC19 plasmid is frequently used as a vector in recombinant DNA procedures to clone genes. The vector carries gene markers for LacZ and ampicillin resistance. Bacteria carrying the LacZ gene produce blue colonies in diagram below) in presence of X-gal. In a given experiment, a gene was inserted into a restriction site in the polylinker of this vector and E. coli cells were then transformed. To determine which cells carried the inserted gene, colonies were grown and replica plated in the following media containing ampicillin and x- gal. The results are as shown: No ampicillin ampicillin ampicillin and X-gal Blue colonies 1 1 2 3 4 5 6 7 8 9 1 2 6 1 6 4 2 4
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a) Which colonies lack the plasmid DNA (native or recombinant)? Why? (6 points) 3, 5, 7, 8, 9. they cannot grow in presence of ampicillin so they do not have the plasmid carrying the antibiotic resistant gene b) Which colonies contain the non recombinant plasmid ? Why? (6 points) 1,2, they are ampicillin resistant and have LacZ gene undisturbed (without the insertion of foreign DNA) c) Which colonies have the recombinant DNA plasmid? Why? (8 points) 4, 6 they are ampicillin resistant and has the LacZ gene inactivated by insertion of foreign DNA, so they are recombinant.
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This test prep was uploaded on 04/16/2008 for the course BIO SCI bis 101 taught by Professor Quiros during the Winter '97 term at UC Davis.

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Bis101_Quiros_Fall2002_Final - BIS101-01 FINAL EXAM ID Fall...

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