Bis101_Quiros_Fall2002_Final

Bis101_Quiros_Fall2002_Final - BIS101-01 FINAL EXAM ID Fall...

Info icon This preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
BIS101-01 FINAL EXAM Fall 2002 NAME: ID# Last First (last 6 digits) Authorization for grade posting by 6 last digits of SS# I,________________________ , ID#(6 last digits)_________________________ authorize posting of my grade in Briggs (1rd floor) by the 6 last digits of my ID# Note: Final exams will not be returned and no re-grading petitions will be honored . Only claims for clerical errors (errors adding points or transferring grades to roster) will be accepted. The exams will be only available for inspection by appointment (from Dec 18-21,contact Bo Yang ( [email protected] ). From Dec 23- Jan 10th come to 116 Asmundson. SHOW ALL WORK TO GET FULL CREDIT .
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
FINAL F’02 QUESTION VALUE SCORE 1 20 2 20 3 24 4 20 5 20 6 20 7 20 8 15 9 25 10 12 TOTAL 200
Image of page 2
1. (a) Tomatoes have a diploid (2n) number of 24 chromosomes. Identify the number of chromosomes that would be expected in a tomato plant that is: 1. 23 a monosomic. (2) 2. 25 a trisomic. (2) 3. 24 a heterozygote for a reciprocal translocation. (2) 4. 22 a nullisomic. (2) 5. 60 a pentaploid. (2) (b). (10 pts) Match the names to the discoveries : ____4 Jacob and Monod 1. Linkage maps ___7_ Benzer 2. Trp operon/attenuation ___9 Morgan 3. Recombinant DNA ___10 Holliday 4. Lac operon ___1 Sturtevant 5. Proof of chromosome theory ___5_ Bridges 6. DNA semi-conservative replication ___6__Meselson-Stahl 7. Cistron ___8__Beadle and Tatum 8.One gene one enzyme ___2__Yanofsky 9. Sex Linkage ____3__ Cohen and Boyer 10. Model for recombination 2. (20 pts) The pUC19 plasmid is frequently used as a vector in recombinant DNA procedures to clone genes. The vector carries gene markers for LacZ and ampicillin resistance. Bacteria carrying the LacZ gene produce blue colonies ( ( in diagram below) in presence of X-gal. In a given experiment, a gene was inserted into a restriction site in the polylinker of this vector and E. coli cells were then transformed. To determine which cells carried the inserted gene, colonies were grown and replica plated in the following media containing ampicillin and x- gal. The results are as shown: No ampicillin ampicillin ampicillin and X-gal Blue colonies 1 1 2 3 4 5 6 7 8 9 1 2 6 1 6 4 2 4
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
a) Which colonies lack the plasmid DNA (native or recombinant)? Why? (6 points) 3, 5, 7, 8, 9. they cannot grow in presence of ampicillin so they do not have the plasmid carrying the antibiotic resistant gene b) Which colonies contain the non recombinant plasmid ? Why? (6 points) 1,2, they are ampicillin resistant and have LacZ gene undisturbed (without the insertion of foreign DNA) c) Which colonies have the recombinant DNA plasmid? Why? (8 points) 4, 6 they are ampicillin resistant and has the LacZ gene inactivated by insertion of foreign DNA, so they are recombinant.
Image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern