Bis101_Quiros_Fall1999_Midterm2

Bis101_Quiros_Fall1999_Midterm2 - NAME Last BIS101-2 Fall...

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NAME: ID# Last, First BIS101-2 Fall 99 MIDTERM 2 SHOW ALL WORK TO GET FULL CREDIT . Authorization for Public Distribution of Graded Examination and grade posting by 6 last digits of SS# I,____________________________ , ID#__________________________ authorize that after this exam is graded, it can be distributed in class or placed in a holding bin, labeled BIS 101-02, on the third floor of Briggs Hall. Also I authorize posting of my grade in the same location by the 6 last digits of my ID#
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MIDTERM 2 QUESTION VALUE SCORE 1 10 2 10 3 12 4 10 5 10 6 10 7 10 8 6 9 10 10 12 TOTAL 100
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1) In an interrupted mating experiment an Hfr E coli prototroph strain transfers three of its genes at the following times to an auxotroph F- strain : Gene from Hfr Time (minutes) Ala+ 5 Phe+ 10 Lac + 12 Leu+ 18 a) What is the order of the genes on the bacterial chromosome starting from the origin of the F factor?: (2) ala phe lac leu b) Assuming that the Hfr strain is sensitive to streptomycin and F- is resistant to this antibiotic, what plate composition would you use to select for lac + leu+ exconjugants? (4) minimal media supplemented with ala and phe, containing streptomycin and lactose as carbon source c) In another interrupted mating experiment a Lac+ bacterial strain with the ability to form a pilus conjugates with a F- Lac- strain. The resulting exconjugants become Lac+ and have the ability to form a pilus. What type of donor was the original Lac+ strain? Explain (4) F' because this plasmid carries a complete F factor plus a few bacterial genes, in this case the lac+ allele . 2) The following biosynthetic pathway leading to the synthesis of product E in a fungus was constructed on the basis of 5 mutants (p to u), each blocking a single step in the pathway. Reconstruct in the table provided the experimental data leading to this result as follows: Enter a “+” for mutant growth and a” -“ for lack of growth for each of the compounds (A to F) added as supplements to the media. ( Hint: mutant p grows only when product E is supplemented) (10)
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   u  →  B t  → C r  → D q  A s  → F p  → E A B C D E F p - - - - + - q + - - - + + r + - - + + + s - - - - + + t + - + + + + u + + + + + + 3. A transduction experiment is carried out involving loci a, d and e. The recipient cell is auxotrophic for a, d and e and the donor is prototrophic for
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This test prep was uploaded on 04/16/2008 for the course BIO SCI bis 101 taught by Professor Quiros during the Winter '97 term at UC Davis.

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Bis101_Quiros_Fall1999_Midterm2 - NAME Last BIS101-2 Fall...

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