Bis101_Quiros_Fall2000_Final

Bis101_Quiros_Fall2000_Final - BIS101-02 FINAL EXAM ID#...

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BIS101-02 FINAL EXAM Fall 2000 NAME: ID# Last First (last 6 digits) SHOW ALL WORK TO GET FULL CREDIT . Code of Academic Conduct All members of the academic community are responsible for the academic integrity of the Davis campus. Existing policies forbid cheating on examinations, plagiarism and other forms of academic dishonesty. Academic dishonesty is contrary to the purposes of the University and is not to be tolerated. Those who violate campus rules regarding academic misconduct are subject to disciplinary sanctions, including suspension and dismissal. Examples of academic misconduct include: 1) Receiving or providing unauthorized assistance on examinations, 2) Using unauthorized materials during an examination 3) Altering an exam and submitting it for re-grading 4) Using false excuses to obtain extensions of time 5) Taking an exam for another student. Authorization for grade posting by 6 last digits of SS# I,____________________________ , ID#_(6 last digits)_________________________ authorize posting of my grade in Briggs (1rd floor) by the 6 last digits of my ID# Note: Final exams will not be returned and no re-grading petitions will be honored. Only claims for clerical errors (errors adding points or transferring grades to roster) will be accepted. The exams will be only available for inspection by appointment at 116 Asmundson until Jan 12th 2001. 1
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FINAL 00 QUESTION VALUE SCORE 1 2 3 4 5 6 7 8 9 10 11 TOTAL 2
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1. (21 pts) The pBR322 plasmid is frequently used as a vector in recombinant DNA procedures to clone genes. The vector carries tetracycline and ampicillin resistance genes used for selection. In a particular experiment, a gene was inserted into a restriction site in the ampicillin resistance gene, and E. coli cells were then transformed. To determine which cells carried the inserted gene, colonies were grown on media without antibiotics and with antibiotics. The results are as shown: + Tetracycline + Ampicillin  + Tetracycline Antibiotic Free  4 9 2 9 8 4 3 2 6 8 5 6 3 2 1 9 7 4 a) Which colonies have neither plasmid nor recombinant DNA? Why? 1,5,7 Unable to grow in presence of antibiotic, they lack plasmid b) Which colonies have plasmid DNA but not recombinant DNA? Why? 2,4,9 Able to grow in tetra and amp. so transgene has not inserted, otherwise amp gene would be disabled c) Which colonies have recombinant DNA? Why? 3,6,8 Only tetra resist. Amp suscept. means that amp gene has been disabled by accepting transgene 3
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2. The cells shown below were all from the same individual. a) (16) Identify the type of cell division and cell division stage depicted in each
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Bis101_Quiros_Fall2000_Final - BIS101-02 FINAL EXAM ID#...

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