chromatography of plant pigments

chromatography of plant pigments - Sam Christy Lab...

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Sam Christy Lab Partners: J. Googins, T. Dunn TA: He LI 12/11/07 Chromatography of Plant Pigments Introduction: Chromatography is a method of separating mixtures into their pure components. With chromatography, you can take an unknown sample of solvent, dab it on TLC paper, and watch it rise up the paper. You then can determine what is in the mixture by how far up the different parts traveled up the paper. Silica-gel column chromatography was also used in this lab to separate the different compounds and elements. This process involves “filtering” the mixture over a fine powder of silica-gel in a tube, or column. With this technique the less polar components come out the bottom first followed by the more-polar components. After you have the two components that have been filtered, use the UV-VIS Spectrometer to determine what the compounds are. Procedure: Step one was to grind the spinach leaves to extract the pigments that is in them. The leaves are dissolved in organic solvents.
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This note was uploaded on 04/17/2008 for the course CHY 123 taught by Professor Professor during the Spring '08 term at University of Maine Orono .

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chromatography of plant pigments - Sam Christy Lab...

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