IID_Dela_Cruz_Jean_Eden_Module10 .pdf - Jean Eden T. Dela...

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Jean Eden T. Dela CruzII-DNovember 24, 2021DATA AND RESULTS (copy the tables below on your answer sheet)AMYLASETest Tube 1 (boiled saliva)Test Tube 2 (unboiledsaliva)Expected observation with Fehling’ssolutionWhen Fehling's solution wasadded to the boiled amylaseand starch solution, thesolution's color stayed blue andan orange-color is formed at thesurface only.When Fehling's solutionwas added to the unboiledamylase and starchsolution, the solution'scolor turned to red orangecompletely.Expected observation withiodine solutionWhen Iodine solution wasadded to the boiled amylaseand starch solution, the solutionturns dark-purple or black.When Iodine solution wasadded to the unboiledamylase and starchsolution, the color black ordark purple starts todisappear.Write down the salivary amylase-catalyzed reaction in this experiment.Denaturization of amylase when boiled and maltose production from starch when catalyzed byamylase.amylaseC6H10O5 + H2OC12H22O11ConclusionMaltose can be produced from starch with the right catalyzation with amylase, when the amylase wasboiled it became denatured reducing its ability to catalyze starch into maltose producing a mildlypositive result when incubated in a water bath at 40°C compared to test tube 2 when it was notheated and was only incubated which provided a perfect temperature for the amylase to catalyze thestarch into maltose which tested positive on the iodine solution (maltose) and negative for starch.

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Term
Spring
Professor
N/A
Tags
Enzyme, Fehling

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