BIBC 102 Final Notes

BIBC 102 Final Notes - BIOCHEMISTRY 102 FINAL EXAM NOTES...

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BIOCHEMISTRY 102 FINAL EXAM NOTES (Spring 2005, Dr. Paul Price) BIOCHEMISTRY 102 FINAL EXAM NOTES (Spring 2005, Dr. Paul Price) *The final exam will be approximately 300 points, and will be divided into 250 points from weeks 6-10 and 5 points from weeks 1-5. The following list gives you a specific description of the areas that will be covered in the final from weeks 6 to 10 of the class, and identifies the relevant portions of the midterm exam notes that will be covered in the final from weeks 1 to 5. 1. Problems from Problem Sets 6-10, or slight variations on these problems, may be on the exam. 2. Know the concepts, explanations, and important facts presented in lecture. 3. Sequence of reactions, Structure of each molecule, Use of ATP, NAD + , FAD, NADPH, etc., and Subcellular localization (cytosol, inner mitochondrial membrane, mitochondrial matrix, etc.) for the following (a) Fatty Acid Oxidation Such as palmitate in a typical cell starting with the formation of fatty Acyl CoA in the cytosol, entry into the matrix via the carnitine shuttle (because CoASH can’t pass through inner mito membrane), and ending with (for palmitate) 8 acetyl CoA molecules in the mitochondria ( handouts 21 and 22 ). Don’t memorize the structure of carnitine itself- just use a box with an OH group attached to it. Know the point of entry in electron transport of the NADH (Complex I) and FADH 2 (Complex II = succinate) generated in FAO and the expected ATP production from each. You do not need to know the additional enzymatic reactions which are required for the complete oxidation of unsaturated fatty acids, but you should know that there will be one less FADH 2 generated in FAO for each C = C double bond. You do not need to know the detailed enzymatic reactions necessary to oxidize odd chain fatty acids, but you should know that such fatty acids are synthesized only in some marine organisms, and that sequential cycles of FAO result in propionyl CoA which is converted into succinyl CoA in three enzymatic steps, the first of which requires biotin and the last vitamin B12. Know the structure of triacylglycerol, the enzymatic step which releases free fatty acids from triacylglycerols in adipose tissue ( TAG lipase), and the form in which free fatty acids are transported in blood to user tissues ( FFA complexed/ attached to albumin) Cytosol matrix (b) Ketone Body Catabolism β-hydroxybutyrate 2 Acetyl CoA ( handout 24 ) liver matrix (c) Ketone Body Biosynthesis 2 Acetyl CoA β-hydroxybutyrate ( handout 24 ) liver matrix (d) Fatty Acid Biosynthesis 2 Acetyl CoA ( cytosolic) CH 3 CH 2 CH 2 C(O)-S-ACP ( handout 26A ) liver cytosol (e) Transport pathway of Acetyl CoA From the mitochondrial matrix to the cytosol; the pathway for the return of oxaloacetate to the mitochondria via malic enzyme and pyruvate. Know that the transfer of acetyl CoA from matrix to cytosol is driven by 1 ATP hydrolysis and that, in the return of oxaloacetate to the matrix, one NADH is converted to NADPH at the
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This note was uploaded on 04/17/2008 for the course BIBC 102 taught by Professor Price during the Spring '02 term at UCSD.

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BIBC 102 Final Notes - BIOCHEMISTRY 102 FINAL EXAM NOTES...

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