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10-19-06 BIMM100 Assign 4p

10-19-06 BIMM100 Assign 4p - BIMM 100 FALL 2006 Assignment...

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BIMM 100 FALL 2006 Assignment 4 1. Name the two techniques that we learned in class used for detecting if a protein or extract containing the protein has DNA binding activity. List the steps of each technique, including necessary controls, and briefly explain the main concept behind each technique. In addition to determining if there is a binding activity present, discuss what other information can be determined from these two techniques. 2. What is the benefit of heterodimeric transcription factors for regulating gene expression? 3. What is the difference between heterodimeric and homodimeric nuclear hormone receptor proteins? Which type is most likely to have a dominant negative effect if it obtains a mutation in its ligand binding domain that prevents association with the ligand?
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4. EMSA was performed using a radiolabeled DNA fragment from the upstream sequence of gene X. This DNA probe was incubated with (+) or without (-) nuclear extract isolated from tissues A (bone), B (lung), C (brain), and D (skin). The DNA- protein complexes were then fractionated on non-denaturing polyacrylamide gels. The
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