10_6_06_BIMM100_assign2_1

10_6_06_BIMM100_assign2_1 - BIMM 100 FALL 2006 Assignment 2...

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BIMM 100 FALL 2006 Assignment 2 1. A) What is the difference between conservative and semi-conservative DNA replication? B) Explain the experiment covered in class that led to the conclusion that DNA replication is semi-conservative. 2. A) Why is DNA synthesis discontinuous? B) List four different general components needed for DNA replication: 4. Fill in the blank DNA replication begins at the ______as _________binds to the dsDNA to separate the duplex. The torsional stress caused by the unwinding of the dsDNA is relieved by ______. Single-stranded regions of the parental strands are then bound by _______, which prevent ssDNA from re-annealing and keeps it in an optimal conformation for copying by DNA polymerase. On each strand, ________ adds a primer, allowing the specific DNA polymerase ________ to extend the primer with dNTPs. To continue DNA synthesis, the specific DNA polymerase _________, along with __________, and ________ take over, allowing DNA replication to be processive. The primer is then removed by _______, and the specific DNA polymerase________ is used to fill in the gap. The last phosphodiester
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10_6_06_BIMM100_assign2_1 - BIMM 100 FALL 2006 Assignment 2...

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