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Chapter_5 - Chapter 5 Proteins Primary Structure Primary...

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1 Chapter 5 Proteins: Primary Structure Primary Structure: amino acid sequence of its polypeptide chain or chains for a protein of n residues, there are 20 n possibilities proteins contain at least 40 residues peptides < polypeptides < titin Protein purification stabilizing proteins pH dissolved in buffer solutions failure to do so could cause their denaturation o to disrupt conformation of a polymer such that it becomes fully unfolded & does not retain significant secondary structure temperature some proteins denatured by high temps purification normally carried out at temps near 0°C presence of degradative enzymes when tissues are destroyed, degradative enzymes are released o including nucleases (enzymes that degrade nucleic acids) and proteases (enzymes that cleave the peptide bonds or proteins) o can be inhibited by adjusting pH or temperature that inactivate them adsorption to surfaces
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