Lecture 17a - LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR THIS WEEK AS IN LECTURE GUIDE 2. RASMOL # 6, CHYMOTRYPSIN: THE ONE AND ONLY RASMOL ASSIGNMENT TO APPEAR ON THE MIDTERM. **** NO OFFICE HRS OR REVIEWS FOR THIS RASMOL UNTIL SUN 10/15! 3. CONFLICT FOR THE MIDTERM EXAM TH 10/19 7:30PM? MEET BRIEFLY AFTER CLASS ON WED 10/4 TO SET THE DAY AND TIME. PERHAPS FRI 10/20 OR ELSE SAT 10/21? FRIDAY’S LECTURE MICHAELIS-MENTEN EQ: RXN RATE INFO FROM SIMPLE MEASUREMENTS OF [S] AND V in EACH ELEMENTARY RATE CONSTANT ASSOCIATED WITH ONE FREE ENERGY BARRIER HOW TO SHOW A CATALYZED RXN ON A DIAGRAM OF G O VS “PROGRESS OF RXN” TODAY CAN WE COMPARE "EFFICIENCY" OF DIFFERENT ENZYMES? USING CONTROLLED INHIBITION TO LEARN ABOUT ENZYMES HOW TO USE THE LINEWEAVER-BURK PLOT TO FIND Vm AND Km 4. CORNELL EXTERN PROGRAM: SHADOW ALUMNI IN THE WORKPLACE MEETINGS 10/4 OR 10/16 HEC AUDITORIUM 5 - 6:15PM. OR CHECK CAREER SERVICES.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
WHAT IS AN “EFFICIENT” ENZYME? TURNOVER NUMBER: NUMBER OF SUBSTRATE MOLECULES CONVERTED TO PRODUCT PER UNIT TIME WHEN ENZYME IS SATURATED WITH SUBSTRATE. IT IS k CAT TURNOVER NUMBER OF SOME ENZYMES CARBONIC ANHYDRASE 600,000/sec ACETYLCHOLINESTERASE 25,000/sec PENICILLINASE 2000/sec CHYMOTRYPSIN 100/sec DNA POLYMERASE I 15/sec LYSOZYME 0.5/sec V MAX = k CAT [E] TOTAL CAN BE ELEMENTARY RATE CONSTANT, OR MORE COMPLEX. IT ANSWERS THE QUESTION: HOW FAST DOES THE ENZYME WORK? e.g. CARBONIC ANHYDRASE , k CAT = 600,000 / sec CONSIDER A SINGLE CARBONIC ANHYDRASE MOLECULE: WHEN ENZYME HAS A SUBSTRATE BOUND, PRODUCT IS FORMED IN 1/ k CAT = 1.7 x 10 -6 SEC LOOK AT TURNOVER NUMBER TABLE:
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/19/2008 for the course BIOBM 3310 taught by Professor Feigenson,gw during the Fall '07 term at Cornell University (Engineering School).

Page1 / 11

Lecture 17a - LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online