36 - LECTURE 36 ANNOUNCEMENTS 1 QUIZ 9 A 23.5 B 24.5 C 23.1...

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LECTURE 36 ANNOUNCEMENTS 1. QUIZ 9: A 23.5 B 24.5 C 23.1 WEDNESDAY’S LECTURE TODAY WHAT HAPPENS IN PLANT PHOTOSYNTHESIS BIOSYNTHESIS OF FATTY ACIDS SO-CALLED "FUTILE CYCLE" GLUCONEOGENESIS EVENTS IN PSII . RAPID TRANSFER OF e - FROM P680*, EVENTUALLY TO MOBILE QUINONE . BUILD-UP OF POSITIVE CHARGE IN THE OEC, THEN OX. OF 2H 2 O . 4H + PUMPED FROM STROMA INTO LUMEN FOR EACH O 2 EVOLVED AT CYT-bf (SIMILAR TO COMPLEX III) . Q-CYCLE OCCURS, WITH H + PUMPING; WATER-SOL. REDUCED PROTEIN PRODUCT, PLASTOCYANIN 2. HOW TO STUDY THIS MASS OF FACTS RE OX PHOS AND PHOTOSYNTHESIS?! USE LAST YEAR'S QUIZZES AS A GUIDE MY OVERALL PLAN IS TO SHOW SOME STEP(S) AND ASK "WHAT HAPPENS?" OR TO ASK, "AT WHICH STEP DOES XXX HAPPEN?" 3. PLAY MAH JONG AND FIGHT HEPATITIS B AT THE SAME TIME. YES. ON SAT NIGHT…. AT PSI e - FROM PLASTOCYANIN USED TO FORM NADPH
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GLUCONEOGENESIS DOWN ARROWS SHOW GLYCOLYSIS UP ARROWS SHOW GLUCONEOGENESIS GLUCONEOGENESIS IS AN ANCIENT PATHWAY AT MOST STEPS: REVERSAL OF GLYCOLYSIS! ( G ~0) i.e. EXACTLY THE SAME RXNS, SAME ENZYMES! THREE STEPS OF GLUCONEOGENESIS ARE IRREVERSIBLE THESE 3 STEPS ARE DIFFERENT FROM THOSE OF GLYCOLYSIS 2C 3 H 8 0 3 + 4ATP + 2GTP + 2NADH + 2H + + 6H 2 O C 6 H 12 0 6 + 4ADP + 2GDP + 2NAD + + 6H + + 6HPO 4 2- PYRUVIC ACID GLUCOSE OVERALL RXN STARTING FROM PYRUVATE :
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GLUCONEOGENESIS - FIRST "BYPASS" OF GLYCOLYSIS RXNS LACTATE LACTATE DEHYDROGENASE MOST GLUCONEOGENESIS OCCURS IN LIVER MAIN CONTROL: ACETYL- CoA IS REQUIRED SIGNAL THAT “FUEL IS ABUNDANT: STORE SOME” ACETYL-CoA REQUIRED FOR ACTIVITY PYRUVATE CARBOXYLASE CORI CYCLE GLUCOSE-ALA CYCLE AMINOTRANSFERASE MITOCHONDRION CYTOSOL PEP IS UNSTABLE: CANNOT MERELY REVERSE THIS RXN INSTEAD, GLUCONEOGENESIS USES UP 2 ATP AND 2 GTP PER GLUCOSE IN A SERIES OF RXNS
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