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Unformatted text preview: 4 8, etc. Isolated proteins and looked at protein expression at 10 minute intervals Oscillating protein levels are related to cell division cycles calls protein cyclin (A and B) Cyclin is always made- oscillation comes from regulated degradation Cyclin is similar to MPF … purify MPF MPF = cyclin + CDC2/28 (aka CDK, cyclin dependent kinase I) CDK is only active when bound to cyclin MASUI Frog eggs- oocyte maturation in vitro In vivo , progesterone leads to meiosis Take egg arrested in metaphase II transfer cytoplasm to G2-arrested oocyte maturation Some factor in cytoplasm induces maturation MPF = maturation promoting factor MPF activity is LOW/G2, HIGH/M1, LOW/I, HIGH/M2, LOWI … etc … oscillates Cyclin high CDK1 on mitosis Cyclin low CDK1 off S G2 M G1 S-cyclin and M-cyclin bind to CDK to start S and M phases- degraded after phase...
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- Fall '07
- Cell Cycle, induces maturation MPF, CDC mutants cells, MPF MPF, factor MPF activity