PCB3063Test1Key - PCB3063 Answer Key Test 1 Name_KEY 1...

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PCB3063 Answer Key Test 1 1 Name:_____ KEY _______________ 1) Indicate whether the following terms relate to Northern blots, Southern blots, or both types of blots (write “NORTHERN”, “SOUTHERN”, or “BOTH”): a) Restriction digest performed before running ______ SOUTHERN ___________ b) Target nucleic acid is denatured before detection ___ SOUTHERN _________ c) Involves hybridization to labeled probe __________ BOTH ________________ d) Can be used to establish where genes are express ___ NORTHERN __________ e) Nucleic acids separated by gel electrophoresis ________ BOTH ____________ f) Intron sequences can be used (successfully) as probes ___ SOUTHERN ______ 2) Compare and contrast the mouse with fungal model systems ( Saccharomyces cerevisiae and Neurospora crassa ) and invertebrate model systems ( Drosophila melanogaster and Caenorhabditis elegans ). Describe both benefits and drawbacks of the mouse relative to the other model system you describe and provide at least one reason that geneticists still use the mouse? Both fungal and invert. animal systems grow very rapidly and produce many progeny . They are very inexpensive to work with. Fungal model systems can often be propagated as a haploid (this is obligatory for Neurospora ) so you don’t have to worry about whether alleles are dominant or recessive. Mouse would still be used because it is a vertebrate and therefore more similar to humans (could also be expressed as genome similar to ours). This similarity to humans is the major benefit of the mouse. Comparisons among the different genome attributes of the various model systems (i.e. genome size, structure, and availability of sequence information). Availability of background information. Another criterion is not referring to any of the above model organisms as being prokaryotes, which would be incorrect.
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PCB3063 Answer Key Test 1 2 3) The biosynthetic pathway for tetrapyrroles in higher plants is shown. Assume that you have mutants that can “turn off” the activity of specific enzymes for experiments (by shifting from one environmental condition to another). The conditions under which the enzyme is off are called “restrictive conditions”. a. Based upon the information shown above, would it be possible for a mutant in haem oxygenase to continue to grow under restrictive conditions if the plant could be supplied with exogenous haem? Explain your answer. No, because it would be unable to make phytochromobilin
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PCB3063 Answer Key Test 1 3 b. Is there a compound that could be supplied that would change your answer to part a of this question? Explain.
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