sanger method - Sahil Amin Professor Shobha Biology 230W 21...

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Sahil Amin Professor Shobha Biology 230W 21 October 2014 Sanger Sequencing Method In biology, DNA sequencing is necessary for proper gene analysis. During the 1970s, Frederick Sanger developed a method of DNA sequencing that revolutioned biology. Dubbed as “Sanger Sequencing,” Sanger’s method became the de facto manner in which to sequence DNA for decades to come. It is only recently that technology has allowed biology laboratories to develop faster methods of DNA sequencing. The Sanger method uses dideoxynucleotide sequencing in order to determine the sequence of DNA. Dideoxynucleotides are different from deoxynucleotides that are found in normal DNA. In normal DNA, there is a hydroxyl (OH) group attached to the 3’ carbon. A dideoxynucleotide does not have this; instead a hydrogen (H) atom is attached at the 3’ carbon. Due to the lack of an OH group, the DNA chain cannot form phosphodiester bonds between all the nucleotides. This results in an early termination of the nucleotide chain.
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