biolab 1 - Michael Bekhit -1- The efficiency of the Enzyme...

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Michael Bekhit -1- The efficiency of the Enzyme Amylase Using Different pH and Temperatures Michael Bekhit February 20, 2007 Section # 5768 Abstract
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Michael Bekhit -2- The purpose of this experiment was to fin the optimal pH and temperature at which the amylase enzyme has the greatest reaction rate. The hypothesis that the optimum temperature for this experiment will be discovered in between the 45-55 ° C range was supported as the optimum temperature was discovered to be 45 ° C. Thus 45 ° C is the temperature where the highest reaction rate occurs without a denaturing of the enzyme. Hence, the enzyme amylase at a temperature less then 45 ° C can reach optimization by increasing in heat. However, at temperatures above 45 ° C the enzyme become denatured due to excessive heat thus losing this optimal reaction status. (Campbell & Reese, 2005). In this experiment the hypothesis of optimal pH was proven incorrect. The hypothesized range was proven to be incorrect in the optimilization of this reaction. Instead of the predicted 5.5-6.0 pH range the true optimal pH is in the 4.0-4.5 pH range. This is because at a higher pH than 4.5 significant changes in the hydrogen bonds adversely affect the facilitation of this enzymatic reaction (Campbell & Reese, 2005). Thus, the optimal pH was discovered to be one of 4.5. This was compared to previously documented information on the enzymatic rate to support one hypothesis yet refute the other. Introduction
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Michael Bekhit -3- Background Chemical reactions are an intricately intertwined in biological and cellular activity, respiration, and survival. However often reactions left to react without help prove energetically inefficient due to time constraints. This time constraint can be lowered by the use of enzyme. An enzyme is a protein which facilitates a chemical reaction more speedily yet does not affect the outcome of the process. An enzyme quickens a chemical reaction by lowering its activation energy. The activation energy can be defined as the amount of energy required to reach the transition state as to facilitate a chemical reaction. Enzyme kinetics provides viable information regarding enzymatic cellular function, and environmental change in regards to this cellular function (Campbell E+S ES E+P whereas E is the enzyme, S is the substrate, ES is the enzyme substrate complex, and P is the product. Through this formula it is absorbed that enzyme substrate complex (ES) breaks down to form product (P) (Vliet, 1993). The type of substrate and several other factors affect the rate at which this process occurs. In this experiment two of these factors the pH and temperature involved with this enzymatic rate will be analyzed in depth. Through experimentation the optimal temperature and pH will be discovered providing
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This note was uploaded on 04/13/2008 for the course BSC 2011 taught by Professor Haas during the Spring '08 term at University of Florida.

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biolab 1 - Michael Bekhit -1- The efficiency of the Enzyme...

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