Bio lab 4 - present, the more substrate there is for the...

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1Anita Ramnath 8. Since the enzyme was heated was 100 E C in tube 5, I would expect it to get denatured and therefore make it incapable of very much activity. 9. Tubes 3b and 3c were both negative controls for the results. It showed us what no reaction would look like because 3b had no sucrose to catalyze, and 3c had no enzyme to control the chemical reaction. The reaction rate increases with temperature until it reaches a maximum level, at which the rate abruptly declines with increasing temperatures. This is because most enzymes get denatured at high temperatures, usually 40 E C for animals. Some enzymes even lose their activity when frozen. Since enzymes are dependent on the presence of the substrate to have activity, the more substrate
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Unformatted text preview: present, the more substrate there is for the enzyme to act upon. Which means the rate at which the substrate is acted upon is dependent on the rate at which the substrates molecules collide with enzymes, which is dependent of the substrate concentration. Extremely high or low pH values normally result in complete loss of activity for most enzymes. Extreme of pH can denature an enzyme so that it can no longer function. They like to be at the optimum, where the enzyme is the most active. pH is also a factor in the stability of enzymes. Like enzyme activity, for each enzyme there is also a region of pH optimal stability. The optimum pH value varies greatly from one enzyme to another....
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