genetics review for exam 1 (in class)

genetics review for exam 1 (in class) - A reversion: change...

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A reversion: change in the same locus that was mutated. ie: gene mutates from “A” to “a” (mutation, induced by mutagens, radiation, mistake, chemicals, etc.), the reversion is when it goes back to “A” Suppression is a second gene “B.” Mutation “B” > “b.” Suppressor mutation is when the “B” suppresses the mutation, and when it’s “b” the mutation is seen. Reporter gene: (no significance to CAT, which is a reporter gene). One uses CAT (or reporter gene) to monitor the activity of the promoter in question. [Question 4 on practice test]: Assume there’s an element between B & C that acts positively on transcription (turning it on). If one graphs the amount of transcription, (approximate numbers) A = 90%, B = 90%, C = D = E = 20% (bar graph). Upstream = 5’ end Pyrosequencing: novel technology, with which you add bases. Take a big strand and one adds a base pair, then another, then another, etc. Whichever dNTP gets added, that base gets incorporated into the strand, and pyrophosphate is released. If dATP doesn’t work, then you try dCTP, then dGTP, then dTTP. (NO de novo,
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genetics review for exam 1 (in class) - A reversion: change...

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