AAA Isolation and restriction of bacterial plasmid DNA

AAA Isolation and restriction of bacterial plasmid DNA -...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
bacterial transformation Abena Asafo-Adjaye October, 2007 Prof. Stahl
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Abstract: This shows how to extract DNA from two bacterial cultures E. coli BL21 and another with a plasmid pGEX-2T in it. The E. coli BL21, acts as the negative and will be able to determine how the plasmid DNA will be treated with a restriction enzyme. The cut and results are seen using agarose gel electrophoresis. The restriction enzyme however must be able to cut at the appropriate allele and because our enzyme did not cut all results look the same. Also results looked as though they were “nicked” due to generous mixing. Introduction: Deoxyribonucleic acid (DNA) is the long term storage information of a cell’s genome, often referred to as the “blueprint” of cells. The DNA has no enzymatic function like proteins do. DNA is a long polymer of simple units called nucleotides, with a backbone made of sugars and phosphate groups. The sequence of the sugars makes up bases, four bases along the backbone that encode information within the DNA. The sugars provide information and are read using the genetic code. The code tells us which amino acid is lined up with the correct protein. The code is read by copying stretches of DNA to what is called the RNA, this is done by transcription. Most
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/17/2008 for the course CHM 101 taught by Professor Johnson during the Spring '08 term at UMass Dartmouth.

Page1 / 4

AAA Isolation and restriction of bacterial plasmid DNA -...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online