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Spectrophotometry Discussion Section

Spectrophotometry Discussion Section - 600nm which are then...

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Discussion: This experiment’s objective was successfully fulfilled by measuring the wavelengths of light that was transmitted, and the results show which wavelengths of light are reflected (transmitted) by each color sample. Where the transmittance is highest, it shows which wavelengths of light will be passed on and seen by a person looking at that color. The transmittance of the red solution was seen to be highest from 600-700nm, while its lowest transmittance was from 400-550nm. The color which corresponds to the red solution’s region of low transmittance and high absorbance is blue. For the blue solution the transmittance was highest from 400-550nm, while the solution’s transmittance was lowest in the 600-650nm range. The color red corresponds to the blue solution’s point of lowest transmittance. When a person is looking at the clear red solution the wavelengths of light below 600nm are being mostly absorbed by the pigment, causing the liquid to transmit the wavelengths above
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Unformatted text preview: 600nm which are then absorbed by the retina in the eye. The red solution absorbs light of the highest energy level because it absorbs light that is mostly below 600nm in wavelength, which is also the range of visible light possessing the greatest amount of energy. The spectrum of the green solution looks as expected because it peaks at 520nm, which is the range of transition from blue to yellow light. Green would be expected to be between these two colors because in pigments, green is the mixture of yellow and blue. The unknown sample is orange colored, and transmits most of the light in the 550-700nm range. This is the range that is expected because orange light has a wavelength of approximately 600nm. The unknown sample’s identity is REPLACE WITH IDENTITY OF SAMPLE. The standard curve prepared for the unknown sample is used to identify a solution’s concentration by examining the absorbance and transmittance of the substance....
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