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StuDocu is not sponsored or endorsed by any college or universityBacterial Transformation Lab ReportConceptual Approaches to Biology for Majors I (Arizona State University)StuDocu is not sponsored or endorsed by any college or universityBacterial Transformation Lab ReportConceptual Approaches to Biology for Majors I (Arizona State University)Downloaded by Selina Lee ([email protected])lOMoARcPSD|11524282
Nguyen2ABSTRACTThe technique of transforming cells such as bacteria in genetic is pertinent for theimprovements of molecular biology. The objectives of this lab are: to perform genetictransformation using the bacterial transformation method, and to observe the results of bacterialtransformation in various growth condition. As mentioned above, the methodology used wasbacterial transformation protocol. Which is a series of technique used to introduce a foreignplasmid into the bacteria (E. Coli), then the bacteria will multiply within the plasmid.If executed properly, the results should display growth and glow under –pGLO with LB, +pGLOwith LB/Amp, +pGLO with LB/Ara, and +pGLO with LB/Ara/Amp. Therefore, leads to theconclusion that bacteria will transform under the right conditions.INTRODUCTIONThe bacteria being transformed in this lab is Escherichia Coli (E. Coli), which is a rod-shaped bacteria that is usually found in the lower intestine of warm-blooded organisms. Theprocess of bacterial transformation revolves mainly around the plasmid needed for bacteria’sgrowth. Within the plasmid, there are genes such as: the AraC protein which will regulate the L-arabinose operons in E. coli, the PBAD promoter that will aid in the transcription of AraC, theGreen Fluorescent Protein (GFP) that will glow under UV light, the Bla gene that codes for theenzyme beta-lactamase which provides bacteria with antibiotics, and the Ori gene that will signalfor the origin of replication.This report analyzes an experiment conducted in order to transform E. Coli using GFP.The null hypothesis is that the E. Coli bacteria will glow and grow the most under +pGLO withLB/Ara. While the alternative hypothesis is that the E. Coli bacteria will glow, and grow themost under +pGLO with LB/Ara/Amp.Bacterial transformation is crucial for in science because it allows geneticallyengineered bacteria to produce medicines. Due to that reason, this lab is interesting because itprovides a deeper understanding of how the topic works, and the lab might result in aninnovative finding about bacterial transformation.

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Term
Winter
Professor
N/A
Tags
Escherichia coli

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