Chapter 11 - DNA Replication and Combination

Chapter 11 - DNA Replication and Combination - Chapter 11...

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Chapter 11 DNA Replication and Recombination DNA is reproduced by semiconservative replication each replicated DNA molecule would consist of one ‘old’ and one ‘new’ strand - conservative replication complementary polynucleotide chains are synthesized, and two newly created strands then come together and parental strands reassociate o original helix is conserved - dispersive replication parental strands are dispersed into two new double helices following replication - Meselson-Stahl Experiment – provided strong evidence that semiconservative replication is the mode used by bacterial cells to produce new DNA molecules o experimental procedure involves use of sedimentation equilibrium centrifugation (density gradient centrifugation) samples are forced by centrifugation through density gradient of heavy metal salt molecules of DNA will reach equilibrium when their density equals the density of the gradient medium - semiconservative replication in eukaryotes o autoradiography common technique that, when applied cytologically, pinpoints location of radioisotope in cell photographic emulsion is placed over histological preparation containing cellular material - origins, forks, and units of replication o origin of replication where DNA replication is initiated along the chromosome DNA is bidirectional proceeds in both directions away from origin o replication fork initially appear at the point of origin of synthesis and then move along DNA duplex as replication proceeds replicon
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Chapter 11 - DNA Replication and Combination - Chapter 11...

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