Chapter 19 - Recombinant DNA Technology

Chapter 19 - Recombinant DNA Technology - Chapter 19...

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Chapter 19 Recombinant DNA Technology Recombinant DNA technology combines several experimental techniques - recombinant DNA combination of DNA molecules that are not found together in nature o molecules produced by joining DNA obtained from different biological science - basic procedures for DNA recombination o DNA to be cloned is purified from cells or tissues o restriction enzymes used to generate specific DNA fragments recognize and cut DNA molecules at specific nucleotide sequences o fragments produced by restriction enzymes are joined together to other DNA molecules that serve as vectors – carrier molecules recombinant DNA molecule vector joined to a DNA fragment o recombinant DNA molecule is transferred to a host cell molecule replicated within host cell produces dozens of identical copies (clones) o recombinant DNA molecules passed on to all progeny within host cell created population of host cells carrying copies of cloned DNA sequence o cloned DNA can be recovered from host cells, purified, and analyzed o cloned DNA can then be transcribed mRNA is translated encoded gene product isolated and used for research or sold commercially recombinant DNA technology is foundation of genome analysis - recombinant DNA and gene-cloning technology provides scientists with methods for isolating large quantities of specific genes or other DNA sequences from large and complex genomes restriction enzymes cut DNA at specific recognition sequences - each restriction enzyme binds to DNA and recognizes recognition sequence specific nucleotide sequence o enzyme cuts both strands of DNA within recognition sequence in specific cleavage pattern - restriction fragments genomic DNA fragments o enzyme cuts this accurately - most recognition sequences contain form of symmetry in which nucleotide sequence read as a palindrome same on both strands of DNA vectors carry DNA molecules to be cloned - restriction fragments cannot directly enter host cells to be copied - vectors – carrier DNA molecules that transfer and replicate inserted DNA fragments o recombinant vectors vectors carrying inserted fragments
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This note was uploaded on 04/17/2008 for the course BIOL 2500 taught by Professor Finger during the Fall '07 term at Rensselaer Polytechnic Institute.

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Chapter 19 - Recombinant DNA Technology - Chapter 19...

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