Unformatted text preview: using a double-headed needle. We will inject 1mL of the emul±on using a syringe F³ed with a 25 G needle into the peritoneal cavity of the mouse. A´er two weeks the intraperitoneal injec±on will be repeated using Incomplete ²reud’s adjuvant. We will then kill the mouse using CO2 asphyxia±on and withdraw blood from the heart. Using a syringe F³ed with 23G needle we will withdraw slowly from the apex of the heart. Transfer blood into a micro centrifuge tube. Es±mate volume and place small wooden rod into the tube & allow the blood to clot at RT incuba±on followed by overnight 4 °C . Remove clot carefully and centrifuge the tube. Transfer serum into a new tube and es±mate volume. We will determine the levels of speciFc an±bodies using ELISA. We will then compare all four groups and analyze the levels of speciFc an±bodies in all four groups to determine the answer to the ques±on stated above....
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- Spring '14
- specific antibodies, TBA protein