microbiolab

microbiolab - Introduction There are two primary reasons it...

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Introduction There are two primary reasons it is important to isolate pure cultures and identify Gram + cocci, particularly in a clinical setting. Number one, the possibility of the existence of the only significantly pathogenic bacterium in the family of Gram+ cocci, namely, Staphylococcus aureus , needs to be excluded for the safety of patients. Further, even other species of bacterium can lead to nosocomial infections that could potentially lead to fatalities of hospital patients – even more so, if they are antibiotic resistant. It is important to maintain highly sterile environments in hospitals. Procedures The experimenter’s nose was swabbed for bacteria and as outlined in page 166 of the lab manual, a blood agar plate was streaked with that bacteria. It was incubated at 35 degrees Celcius for 24 hours. Three isolated colonies from the 3 rd quadrant were Gram stained, as explained on page 43 of the lab manual. Next, a colony was inoculated onto a streak plate to isolate a pure culture and again incubated at 35 degrees Celcius for 24 hours. Streaking procedures followed are explained on pages 57-59 of the lab manual). As mentioned below in results the Gram stain was purple with round grape-like clusters with a circular colony with a radius of 0.5 mm, white, and glistening. . List of Media and Tests Run: 1. Blood Agar Plates 2. Mannitol Salt Plate 3. DNAse Plate and Test 4. Mueller-Hinton Plate 5. Oxidase Test 6. Catalase Test 7. Mannitol Salt Growth and Fermentation Tests 8. Antibiotic Sensitivity Test
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Results Subculture Gram Stain – Gram Positive, Clusters, Cocci
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This note was uploaded on 04/17/2008 for the course BIOL 402 taught by Professor Faurot during the Spring '08 term at Kansas.

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microbiolab - Introduction There are two primary reasons it...

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