07sexam3 - Microbiology Biology Laboratory Biology 203/402...

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Microbiology Biology Laboratory Name ________________ Biology 203/402 Third Examination TA’s_________________ S pring, 2007 Multiple Choice: Answers may be used more than once a. Salmonella b. Shigella c. Escherichia d. Streptococcus e. Staphylococcus 1. A basketball player, sharing a locker room with many other players, suffered a multi-drug resistant __________________ infection in an open scrape wound on his shin. This type of infection is referred to as community acquired. The organism was a Gram positive coccus in clusters, beta hemolytic on blood agar, catalase positive, and DNase positive. 2. In a multi-state outbreak in 2004 , several people became ill with a diarrheal illness following consumption of undercooked beef. The supplier had followed Food Safety and Inspection guidelines, and there were no product recalls. However, the beef contained ________________, a Gram negative rod which did not ferment lactose. When grown on Hektoen agar, black centered colonies were observed. 3. Several cases of gastroenteritis, including diarrhea and fever, occurred in 2004 in petting zoos in North Carolina, Florida and Arizona. Cultures confirmed the gel electrophoresis findings. Cultures indicated a Gram negative, lactose fermenting rod. The organism was also methyl red positive, and Voges Proskauer negative. 4. Jim Henson, creator of the Muppets, died of pneumonia at age 53 from an untreated _________________ infection. This organism was a Gram positive coccus, catalase negative, and Group A postive by a latex agglutination test. 5. In 2004, of the 16,000 foodborne infections in the US, 2,230 were associated with fecal contaminated foods containing the Gram negative rod _______________. This organism does not ferment lactose, does not survive selenite selection, and is non-motile. Multiple Choice: Most bacteria found in urine are due to contamination by normal skin flora during passage of urine. The presence of bacteria in urine is not considered a urinary tract infection unless at least 10 5 CFU/ml of urine are found. Mary visited Watkins suffering from a burning sensation upon urination. A 0.1 ml volume of her urine was transferred to a 9.9 ml saline dilution blank (Tube 1). After mixing, 1.0 ml was transferred from Tube 1 to a 9.0 ml dilution blank (Tube 2).
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07sexam3 - Microbiology Biology Laboratory Biology 203/402...

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