3-4 Simple Stain - Experiment 3-4 Smear Preparation(pgs...

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1 Experiment 3-4: Smear Preparation (pgs. 77-83) Purpose : To learn the proper procedure for making a smear, which will help in later labs when staining bacteria. An improperly made smear could allow the bacteria to wash off the slide or be too thick so bacteria is stacked on top of one another or be so distorted that one can not see the proper shape of the bacteria Materials and Methods: Prepare a bacterial smear from both a liquid culture and plate using the bacteria from Ex. 2-1. Staining alone allows one to see the cellular structures of bacteria. Stains are not successful if a successful smear is not prepared Smears should : - withstand washings - not be too thick - not result in excessive distortion of the organism Smear preparation from plates and solid media : Colony – an aggregate of millions of cells that come from one cell Procedure : 1. Clean slide and draw a target circle on the bottom of the slide 2. Flame the loop and then place a loopful of water on the slide 3.
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