1-1 Culture Media Prep

1-1 Culture Media Prep - Experiment 1-1: (pgs. 9-13) Read...

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1 Experiment 1-1: ((pgs. 9-13) Read ONLY!! Culture Media Preparation/ Complex and Defined Media (Handout) Purpose : To learn the process of making media and the important factors needed for media. Media : food that is used for culturing bacteria and other microorganisms Three Consistencies of Media: 1. Liquid 2. Solid 1.5% Agar concentration 3. Semisolid 0.4 ~ 0.5% Agar concentration Solid and semisolid media are made by adding varying concentrations of a solidifying agent. A good solidifying agent : 1. Is not utilized by the microorganisms 2. Doesn’t inhibit bacterial growth 3. Doesn’t liquefy at room temperature Gelatin is sometimes used as a solidifying agent, but is is hydrolyzed by many organisms and liquefies at room temperature. Agar (made from seaweed) and silica gel are good choices for solidifying agents. Nutrients : chemical compounds that can be broken down into or used to make monomers macronutrients : nutrients required in large amounts: ex. C, N, P, S, K, Mg, Ca, Na, Fe micronutrients : nutrients required in small amounts: ex. Metals Nutritional Requirements : 1. water – all organisms are ~ 75% water - solvent for all chemical reactions - provides an environment for molecular movement 2. carbon – provides a source of carbon - autotrophs – can utilize the carbon in CO 2 to synthesize all materials - heterotrophs – must have one or more organic compounds for carbon
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This note was uploaded on 04/17/2008 for the course BIO 1510 taught by Professor Rodriguez during the Fall '08 term at Wayne State University.

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1-1 Culture Media Prep - Experiment 1-1: (pgs. 9-13) Read...

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