3-9 Endospore Stain

3-9 Endospore Stain - Experiment 3-9: Spore Staining: Two...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Experiment 3-9: Spore Staining: Two Methods (pgs.97-99) Purpose : To learn the proper procedure for staining endospores The spore stain is a differential stain used to detect the presence and location of spores in bacterial cells. Several bacteria, especially ones of the genus Bacillus and Clostridium , produce structures called endospores which are differentiated cells that exist within the bacterial cell (also called the vegetative cell ). Endospores have several layers including the exosporium (the outermost layer; made of the protein keratin which resists staining), the spore coat, the cortex, and the core. The core contains the normal features of a bacterial cell. Differences between the vegetative cell and the core : - complex of dipicolinic acid and Ca 2+ makes up 10% of the dry weight of the core of the endospore - dehydrated nature of the core which only contains about 10 to 25% water - the slightly acidic nature of the core An endospore is a dormant form of the bacterium that allows it to survive poor environmental conditions. Spores are formed when regular cell growth stops due to depletion of nutrients, adverse conditions, etc. The spore can germinate and a new vegetative cell can grow if conditions return to normal. Endospores are resistant to many of the things that normal bacteria are susceptible to – heat, drying,ultraviolet radiation, and chemicals (including dye). The resistance is
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/17/2008 for the course BIO 2200 taught by Professor Choong-minkang during the Fall '08 term at Wayne State University.

Page1 / 2

3-9 Endospore Stain - Experiment 3-9: Spore Staining: Two...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online