7-3 Antimicrobial Susceptibility Test

7-3 Antimicrobial Susceptibility Test - Experiment 7-3:...

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1 Experiment 7-3: Antimicrobial Susceptibility Test (Kirby-Bauer Method)(pgs. 242-244) Purpose : To determine the sensitivity or resistance of a bacterium to different antibiotics Antibiotics are chemical substances produced by microorganisms that inhibit or kill other microorganisms. The Kirby-Bauer Method is a standardized method of testing the effectiveness of antibiotics on bacteria. The Kirby-Bauer Method utilizes Mueller Hinton Agar . This agar is prepared to meet the standard requirements of the Kirby-Bauer test. Standard requirements of Mueller Hinton Agar: 1. pH between 7.2 and 7.6 2. thickness of 4mm in a 100mm petri dish 3. 25ml of media per plate in a 100mm petri dish These standard requirements must be adhered to when preparing the media since a thicker plate would slow the rate of diffusion of the antibiotics in the media, and a thinner plate would increase the rate of diffusion. 1
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7-3 Antimicrobial Susceptibility Test - Experiment 7-3:...

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