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Unformatted text preview: 1 Experiment 3-6: Gram Staining (pgs. 86-90) Purpose : To learn the procedure for doing the Gram stain Materials and Methods: TSB cultures of : Stain/Reagent: Escherichia coli Crystal Violet Staphylococcus epidermidis Grams Iodine Decolorizer (95% Ethanol) Safranin The Gram Stain was developed in the late 1800s by Christian Gram, and it allows bacteria to be classiFed into two groups, ones that were Gram-positive (appear purple) and ones that were Gram-negative (appears the color of the counterstain, in this case red). It is the most important and widely used microbiological differential stain. It is important to use proper technique in performing this procedure in order to have reliability when staining an unknown bacterium. Procedure: 1. Make a smear of the organisms E. coli and S. epidermidis . 2. Make Fve slides - one for each step of the Gram Stain procedure, including one slide of the entire Gram Stain procedure and one slide with just safranin. Gram Stain Procedure : Cover the smears with crystal violet for 1 minute. Briey rinse off the stain. Drain off the excess water....
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This note was uploaded on 04/17/2008 for the course BIO 2200 taught by Professor Choong-minkang during the Fall '08 term at Wayne State University.
- Fall '08